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KMID : 0364819860240020079
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Korean Journal of Microbiology
1986 Volume.24 No. 2 p.79 ~ p.85
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Studies on the Organization and Expression of tRNA Genes in Aspergillus nidulans(Ⅴ)
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Lee Byeong-Jae
Kang Hyen-Sam
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Abstract
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1
We have determined the sequence of tRNAA19 of A. nidulans partially by enzymatic rapid
RNA sequencing technique. The sequence was 5´GGCCGGCUGG000AAXUGGCAAGGXUCUGAXUACGAAXCAGGAGAUUGCACXXXXXGAGCXXUXXGUCGGUCACCA3´. The clover
leaf structure was made from above data. As a result, the anticodon sequence was identified as ACG. This result was confirmed with charging test. The complete sequence was proposed by supplementing the DNA sequence to and by assigning the position of minor bases to this RNA sequence.
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KEYWORD
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